The Kangaroos are presently aligned with both Werribee and North Ballarat, a deal which sees players not selected in the AFL team shared between the two VFL clubs.
From 2016 onwards, they will only appear for the Tigers.
The abandonment of the North Ballarat alignment follows the new involvement of the Western Bulldogs in Ballarat, with plans for premiership matches at Eureka Stadium.
Under the new agreement Werribee will retain its name, nickname, colours, theme song and home ground.
North Melbourne director of football Geoff Walsh said, "Both Werribee and North Melbourne will benefit greatly from the full alignment and we are sure that not only will this decision enhance the football programs of both clubs but the joint approach will further imprint our game in the Western region."
John Lamont will be the senior coach until the end of the 2017 season.
Lamont, who was a development and line coach at Arden Street for seven years before taking on the role at Werribee, said, "The relationship between the two clubs is very strong, very healthy and has produced good results.
"Werribee has developed players like Ben Brown and Luke McDonald that North has drafted, and we have seen North-listed players like Mason Wood (29 games) and Daniel Currie (30 games) hone their skills playing for Werribee, all to the benefit of the North Melbourne Football Club."
