The two-time reigning Dockers best and fairest and 2014 AFL Players' Association MVP hurt his right shoulder attempting a mark in an International Rules practice match in Western Sydney last Tuesday.
Fyfe was due to represent Australia in the Virgin Australia International Rules Test at Paterson Stadium on Saturday but he was withdrawn after scans last Wednesday revealed he had strained his AC joint.
Fremantle sent the 23-year-old to a specialist for further assessment last week and the club was advised on Monday that Fyfe required minor surgery on the joint.
The midfielder will have the minor procedure later this week.
Fyfe had surgery on his left shoulder twice in 2011 and 2012 although the nature of this injury is significantly different.