The $250,000 will be split between three organisations – the AFLPA Past Player Health Hardship Fund ($100k), the Ladder Program ($100k) and RecLink ($50k).
RecLink will use its donation to fund the creation of leagues and teams in outback Australia, currently in the Northern Territory but to come in Western Australia as well.
Former AFL umpire Adrian Panozzo is the CEO of RecLink and said the donation would allow his organisation to do a significant amount of work.
"I suppose when you look at $50,000 in football, a lot of people would assume that might not go a long way, but I can only reassure the Players' Association that in the RecLink football world, that can actually change lives and change communities," he said.
"When you're six, eight or 10 hours away from Alice Springs or Tennant Creek, if you choose to play a game, that might mean you have to leave your community for days.
"What we know is by starting 18 teams, and that's what we can achieve this year with this money, we're actually able to create identity in communities, that we're actually able to restore pride in these communities.
"They might only have a couple of hundred people, but for them to actually develop an identity and wear their local football jumper is such a powerful thing."
Former North Melbourne player Frank Goode, a member of the AFLPA Past Player Membership Advisory Board, accepted the money on behalf the health fund.
"There are a lot of past players that do experience some hardship and this will go a long way to assisting them," he said. "A lot of them need new joints and things like that to keep going so this is fantastic and I thank the current players."
AFLPA CEO Brendon Gale said it was fitting for the health fund to receive some of the funds given the focus of the match.
"It's appropriate that, given it is the Hall of Fame Tribute Match, to pay tribute to those who've made such wonderful contributions to our game that we continue to support those generations in area of health-related difficulty."
The Ladder Program works in the area of youth homelessness and Brisbane Lions co-captain Luke Power said the program was one he was happy to get behind.
"One hundred thousand dollars is to be donated to the Ladder Program," he confirmed. "Youth homelessness is obviously a major problem in Australia, and this program aims to do something about this by building and operating facilities which will provide housing, education and mentoring."
