ASADA representatives explained the investigation process to the players on Wednesday, and also briefed them about their rights and entitlements.
Essendon chairman, David Evans, said in a statement this was an important step forward in helping to resolve the matters before the club.
"ASADA outlined the process for the investigation to continue, including interviews with players to determine if a prohibited substance had been administered," he said.
He stressed that the club was still subject to, and fully cooperating with the ASADA investigation.
"I know our members and the football community want answers, and I ask that the club be allowed time to cooperate with the ongoing investigation."
