Grimes, 21, left training on Friday complaining of soreness in his hamstring.
The defender has been plagued by hamstring problems since being selected by the Tigers with pick 2 in the 2009 pre-season draft.
He has suffered hamstring injuries in the last two Dreamtime at the 'G matches.
In 2011, Grimes suffered a season-ending hamstring injury in the Dreamtime match against the Bombers.
In that instance, Grimes tore his hamstring off the bone.
Report says hamstring injuries on the slide
Grimes has played 17 games for the Tigers, including nine in 2012. His last game was in round 14 against Adelaide.
He travelled to Germany in July with the Tigers' strength and rehabilitation coach, Terry Condon, spending a week with world-renowned soft-tissue specialist Doctor Hans-Wilhelm Muller-Wohlfahrt.
The treatment involved Grimes being injected with actovegin, an extract of calf’s blood, and anti-inflammatory traumeel, in an effort to stimulate muscle repair.
It was similar treatment to that experienced by former Richmond midfielder Mark Coughlan and Geelong premiership player Max Rooke.
Despite the setbacks, Grimes is highly rated across the AFL and won nomination for the NAB AFL Rising Star award in round two.
The Tigers reported Grimes had been training strongly so far this pre-season before Friday's hiccup.
Grimes' brother Jack is co-captain of Melbourne.
