Burns pulled up with calf soreness following a training session on Saturday morning and has withdrawn from the team.
It's a bitter blow for the onballer, who was poised to become the 11th player in Collingwood's history to play 250 games for the club.
Collingwood is yet to confirm who will replace Burns in the 22, however, vice-captain Josh Fraser will lead the side as it looks for its third win from four games.
